| CEACAM8

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) also known as CD66b (Cluster of Differentiation 66b), is a member of the carcinoembryonic antigen (CEA) gene family. Its main function is cell adhesion, cell migration, and pathogen binding.CEACAM8 is expressed exclusively on granulocytes and used as granulocyte marker.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	2.21	1.48	1.03	0.69	0.41	0.3	0.19	0.05
Corrected OD	2.16	1.43	0.98	0.64	0.36	0.25	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of human CEACAM8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	97
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of human CEACAM8 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-94	88-96	90-111
	Average (%)	90	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108